TOYOPEARL AF-Tresyl-650M is an activated resin that is highly reactive toward amine and thiol groups. It is provided in dry form, ready for reaction in buffered solutions containing the ligand to be coupled. Coupling is accomplished in a neutral to slightly alkaline (pH 7 - 8) solution. Under such conditions even proteins of limited stability may be successfully coupled. Coupling leads to the formation of a highly stable secondary amine or thio-ether linkage.
TOYOPEARL AF-Epoxy-650M is an activated resin with a high density of epoxy-functionality, packaged in dry form. I can be used to immobilize proteins or low molecular weight ligands. It is particularly useful when high densities of low molecular weight ligands must be attached. It is a highly versatile starting material for conversion to other chemically active functional groups and may be readily activated to hydrazide-bearing materials.
TOYOPEARL AF-Epoxy-650M is particularly useful for immobilization of carbohydrates or glycoproteins.