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TOYOPEARL Activated Affinity Resins

TOYOPEARL affinity resins are composed of hydrophilic, dimensionally stable base resins that exhibit excellent pressure-flow characteristics. These resins use the TOYOPEARL HW-65 SEC resin as a base bead.

TOYOPEARL affinity resins are functionalized with chemically active groups or group-specific ligands. Resins with activated functional groups are ready to directly couple a protein or other ligand. Resins with reactive groups require carbodiimide coupling or reductive amination to achieve a stable covalent linkage. 

 

TOYOPEARL Activated Affinity Resins are shipped ready for coupling to your ligand of choice directly out of the bottle. While Epoxy resins are recommended for coupling of low molecular weight ligands, Tresyl resins can be applied for coupling of high molecular weight ligands, such as proteins.

TOYOPEARL AF-Tresyl-650M
TOYOPEARL AF-Epoxy-650M

TOYOPEARL AF-Tresyl-650M is an activated resin that is highly reactive toward amine and thiol groups. It is provided in dry form, ready for reaction in buffered solutions containing the ligand to be coupled. Coupling is accomplished in a neutral to slightly alkaline (pH 7 - 8) solution. Under such conditions even proteins of limited stability may be successfully coupled. Coupling leads to the formation of a highly stable secondary amine or thio-ether linkage.

 

AFC_Tresyl_structure.jpg

TOYOPEARL AF-Epoxy-650M is an activated resin with a high density of epoxy-functionality, packaged in dry form. I can be used to immobilize proteins or low molecular weight ligands. It is particularly useful when high densities of low molecular weight ligands must be attached. It is a highly versatile starting material for conversion to other chemically active functional groups and may be readily activated to hydrazide-bearing materials.

TOYOPEARL AF-Epoxy-650M is particularly useful for immobilization of carbohydrates or glycoproteins.

 

AFC_Epoxy_structure.jpg

Choose your product
showing 5 of 5 products
Technology
Affinity (5)
Support
HW (5)
Function
AF-Epoxy (3)
AF-Tresyl (2)
Pore Size
1000 Å (5)
Particle Size
65 µm (5)
Reference TH-08000
Description AF-Epoxy-650 M polymer 65 µm 10 g
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Reference TH-08038
Description AF-Epoxy-650 M polymer 65 µm 100 g
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Reference TH-14471
Description AF-Tresyl-650M polymer 65 µm 5 g
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Reference TH-14472
Description AF-Tresyl-650M polymer 65 µm 100 g
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Reference TH-43402
Description AF-Epoxy-650 M, 65 µm 5g,
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showing 5 of 5 products