Cromatografía de Proteína A

Protein A Chromatography

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The ultra-high capacity TOYOPEARL AF-rProtein A HC-650F exceeds all other commercially available protein A media with regard to its IgG binding capacity of more than 65 g IgG/L resin at residence times of 5 minutes. Its IgG capacity still exceeds 50 g/L at 2 minutes residence time with feed stock concentrations from 1.0 g/L to 10.0 g/L. Improved mass transfer characteristics allow it to maintain a larger percent of its capacity at lower residence times relative to agarose based, caustic stable resins.

The standard TOYOPEARL AF-rProtein A-650F resin binds human and mouse immunoglobulin G, IgM, and Fab fragments. It provides a static IgG binding capacity of more than 45 g/L and a typical dynamic IgG binding capacity of more than 30 g/L at 2 minutes residence time (1 g/L protein load). Fast mass transfer kinetics support high binding capacities at high flow rates.

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Reference TH-22803

Description TOYOPEARL HW-AFC PROCESS RESIN S AF-rProtein A-650F, 45 µm, 9027 90 00 .000 Å, polymer, 10 ml